Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
نویسندگان
چکیده
We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).
منابع مشابه
Large-Scale Expansion of Human Embryonic and Induced Pluripotent Stem Cells for Cell Therapy Applications
Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...
متن کاملIsolation and long-term culture of neural stem cells from Acipenser persicus (Borodin, 1897)
In the present study, an in vitro brain cell culture was developed from neural cells of Persian sturgeon (Acipenser persicus). The tissue samples collected from the anterior, middle and posterior regions of the brain were cultivated separately in DMEM/F12 medium supplemented with 15% fetal bovine serum, antibiotic and antimycotic. The medium was refreshed every 3 days. The cells became confluen...
متن کاملIsolation and long-term culture of neural stem cells from chondrostei fish Acipenser persicus
In the present study, an in vitro brain cell culture was developed from Persian sturgeon (Acipenser persicus). The tissues from anterior, middle and posterior regions of the brain were dissected, dispersed and cultivated separately in DMEM/F12 medium supplemented with 15% fetal bovine serum, antibiotic and antimycotic. The medium was changed every 3 days. The cells became confluent after about ...
متن کاملFingolimod Enhances Oligodendrocyte Differentiation of Transplanted Human Induced Pluripotent Stem Cell-Derived Neural Progenitors
Multiple sclerosis (MS) is an autoimmune disease which affects myelin in the central nervous system (CNS) and leads to serious disability. Currently available treatments for MS mainly suppress the immune system. Regenerative medicine-based approaches attempt to increase myelin repair by targeting endogenous progenitors or transplanting stem cells or their derivatives. Fingolimod exerts anti-inf...
متن کاملFingolimod Enhances Oligodendrocyte Differentiation of Transplanted Human Induced Pluripotent Stem Cell-Derived Neural Progenitors
Multiple sclerosis (MS) is an autoimmune disease which affects myelin in the central nervous system (CNS) and leads to serious disability. Currently available treatments for MS mainly suppress the immune system. Regenerative medicine-based approaches attempt to increase myelin repair by targeting endogenous progenitors or transplanting stem cells or their derivatives. Fingolimod exerts anti-inf...
متن کامل